Origin of the HIV-1 group O epidemic in western lowland gorillas.

نویسندگان

  • Mirela D'arc
  • Ahidjo Ayouba
  • Amandine Esteban
  • Gerald H Learn
  • Vanina Boué
  • Florian Liegeois
  • Lucie Etienne
  • Nikki Tagg
  • Fabian H Leendertz
  • Christophe Boesch
  • Nadège F Madinda
  • Martha M Robbins
  • Maryke Gray
  • Amandine Cournil
  • Marcel Ooms
  • Michael Letko
  • Viviana A Simon
  • Paul M Sharp
  • Beatrice H Hahn
  • Eric Delaporte
  • Eitel Mpoudi Ngole
  • Martine Peeters
چکیده

HIV-1, the cause of AIDS, is composed of four phylogenetic lineages, groups M, N, O, and P, each of which resulted from an independent cross-species transmission event of simian immunodeficiency viruses (SIVs) infecting African apes. Although groups M and N have been traced to geographically distinct chimpanzee communities in southern Cameroon, the reservoirs of groups O and P remain unknown. Here, we screened fecal samples from western lowland (n = 2,611), eastern lowland (n = 103), and mountain (n = 218) gorillas for gorilla SIV (SIVgor) antibodies and nucleic acids. Despite testing wild troops throughout southern Cameroon (n = 14), northern Gabon (n = 16), the Democratic Republic of Congo (n = 2), and Uganda (n = 1), SIVgor was identified at only four sites in southern Cameroon, with prevalences ranging from 0.8-22%. Amplification of partial and full-length SIVgor sequences revealed extensive genetic diversity, but all SIVgor strains were derived from a single lineage within the chimpanzee SIV (SIVcpz) radiation. Two fully sequenced gorilla viruses from southwestern Cameroon were very closely related to, and likely represent the source population of, HIV-1 group P. Most of the genome of a third SIVgor strain, from central Cameroon, was very closely related to HIV-1 group O, again pointing to gorillas as the immediate source. Functional analyses identified the cytidine deaminase APOBEC3G as a barrier for chimpanzee-to-gorilla, but not gorilla-to-human, virus transmission. These data indicate that HIV-1 group O, which spreads epidemically in west central Africa and is estimated to have infected around 100,000 people, originated by cross-species transmission from western lowland gorillas.

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عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 112 11  شماره 

صفحات  -

تاریخ انتشار 2015